Difference Between Gram Positive And Gram Negative Bacterial Cell Wall Pdf
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- Gram Positive vs. Gram Negative Bacteria
- A Comparison of the Mechanical Properties of Different Bacterial Species
- Gram-Positive Bacteria Explained in Simple Terms
Applied Microbiology pp Cite as.
Gram Positive vs. Gram Negative Bacteria
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Start on. Show related SlideShares at end. WordPress Shortcode. Published in: Education. Full Name Comment goes here. Are you sure you want to Yes No. Prachi Verma. Show More. No Downloads. Views Total views. Actions Shares. No notes for slide. Christian Gram, a Danish Physician in developed a staining technique to distinguish two types of bacteria.
The two categories of bacteria based on gram staining are Gram positive bacteria and Gram negative bacteria. Bacteria are first stained with crystal violet or gentian violet. All bacterial cells will stain blue or purple colour with crystal violet solution. Gram negative bacteria are later stained with safranin or fuchsin for observation under microscope.
Gram negative bacteria after safranin or fuchsin staining colour red or pink colour. Gram staining differentiates bacteria by the chemical and physical properties of their cell walls by detecting the properties of peptidoglycan.
Gram staining method is useful in differentiating majority of bacterial species into two broad categories. Even though all bacterial species cannot be differentiated based on gram staining technique, this method has immense application in clinical diagnostics and biological researches. You just clipped your first slide!
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A Comparison of the Mechanical Properties of Different Bacterial Species
Gram-Positive Bacteria Explained in Simple Terms
Bacterial Cell Wall : The anatomy of bacterial cell structure. Bacterial cells lack a membrane bound nucleus. Their genetic material is naked within the cytoplasm.
Peptidoglycan or murein is a polymer consisting of sugars and amino acids that forms a mesh-like layer outside the plasma membrane of most bacteria , forming the cell wall. Attached to the N -acetylmuramic acid is a peptide chain of three to five amino acids. The peptide chain can be cross-linked to the peptide chain of another strand forming the 3D mesh-like layer. Peptidoglycan is also involved in binary fission during bacterial cell reproduction.
Handbook of Electroporation pp Cite as. The bacterial envelope is a complex layered structure, whose primary function is to protect the cell from the environment. The Gram-staining procedure has been a fundamental method to classify the bacteria for more than years. It was based on the effect of the structure and composition of the bacterial envelope on the staining procedure. It has made it possible to classify the bacteria in two main categories: the Gram-positive, which bind the Gram stain, and Gram-negative bacteria, which do not.
Most bacteria are classified into two broad categories: Gram positive and Gram negative. These categories are based on their cell wall composition and reaction to the Gram stain test. The Gram staining method, developed by Hans Christian Gram , identifies bacteria based upon the reaction of their cell walls to certain dyes and chemicals. The differences between Gram positive and Gram negative bacteria are primarily related to their cell wall composition. Gram positive bacteria have cell walls composed mostly of a substance unique to bacteria known as peptidoglycan , or murein. These bacteria stain purple after Gram staining.
It is important to note that not all bacteria have a cell wall. The two different cell wall types can be identified in the lab by a differential stain known as the Gram stain. Originally, it was not known why the Gram stain allowed for such reliable separation of bacterial into two groups. Once the electron microscope was invented in the s, it was found that the staining difference correlated with differences in the cell walls. Here is a website that shows the actual steps of the Gram stain.
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